ARP1 Homolog A antibody

Antigen: ARP1 Homolog A

Synonyms: ARP1, CTRN1, FLJ52695, FLJ52800, FLJ55002, Arp1, alpha-Arp1, ACTR1A, actr1a, MGC83436, arp1, ctrn1, centractin

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185219

Additional Information

Gene ID: 10121, 54130 (mouse)

Immunogen: C-YEEDGARSIHRKT

Format: Affinity-purified

Description: All names and symbols: ACTR1A, ARP1, ARP1 actin-related protein 1 homolog A, centractin alpha (yeast), actin-RPV, centractin alpha, ARP1, yeast homolog A, centrosome-associated actin homolog, ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha). Official Symbol: ACTR1A. RefSeq number(s): NP_005727.1. Gene ontology terms: vesicle-mediated transport, microtubule associated complex, dynactin complex

Specificity: This epitope was selected to minimise the chance of cross-reactivity with ACTR1B (YEEDGSRAIHRKT). Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx. 45kDa band observed in Human Kidney lysates (calculated MW of 42.6kDa according to NP_005727.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185219 staining (2µg/ml) of human kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Lees-Miller, Helfman, Schroer: "A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility." in: Nature, Vol. 359, Issue 6392, pp. 244-6, 1992 (PubMed).