Differentially Expressed in FDCP 6 Homolog (Mouse) (DEF6) antibody

Antigen: Differentially Expressed in FDCP 6 Homolog (Mouse) (DEF6)

Synonyms: IBP, SLAT, SWAP70L, DEF6, MGC157235, Def6, MGC63721, ibp, slat, swap70l, MGC68775

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185227

Additional Information

Alternative name: IBP / DEF6

Gene ID: 50619, 23853 (mouse)

Immunogen: ALRKELLKSIWYA-C

Format: Affinity-purified

Description: All names and symbols: DEF6, IBP, differentially expressed in FDCP 6 homolog, RP3-329A5.5, SLAT, IRF4-binding protein, OTTHUMP00000016251, differentially expressed in FDCP 6 homolog. Official Symbol: DEF6. RefSeq number(s): NP_071330.3

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx. 70kDa band observed in Human Thymus lysates (calculated MW of 73.9kDa according to NP_071330.3). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185227 (1µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Tanaka, Bi, Kitamura et al.: "SWAP-70-like adapter of T cells, an adapter protein that regulates early TCR-initiated signaling in Th2 lineage cells." in: Immunity, Vol. 18, Issue 3, pp. 403-14, 2003 (PubMed).