Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody

Details for Product No. ABIN185240
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Antigen
Synonyms
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, gl ... show more
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, glyceraldehyde-3-phosphate dehydrogenase C2, BEST:GH12586, CG12055, Dmel\\CG12055, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, KNC-NDS6, G3PD, cb609, mg:bb02e05, wu:fb33a10, wu:ft80f05, G3PDH show less
Epitope
C-Term
(82), (37), (15), (13), (10), (9), (8), (7), (7), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(400), (198), (167), (90), (42), (32), (29), (26), (18), (18), (17), (14), (12), (10), (9), (9), (8), (8), (7), (6), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Goat
(271), (193), (33), (18), (16)
Clonality
Polyclonal
Conjugate
Un-conjugated
(31), (28), (11), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(467), (210), (135), (82), (75), (65), (31), (30), (29), (19), (15), (10), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Pubmed 6 references available
Quantity 100 μg
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Catalog No. ABIN185240
374.27 $
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Immunogen Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Specificity GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GAPDH
Background Synonyms: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Gene ID 2597
NCBI Accession NP_002037
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 35 kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1 kDa according to NP_002037). Recommended concentration: 0.01-0.03 µg/mL.
IHC: In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed. Recommended concentration 0.3 µg/mL (data not shown).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody ABIN185240 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody (2) ABIN185240 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Staining of germinal centre cells. B) Staining of endothelial cells.
Product cited in: Nguyen, Pace, Koller: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, 2007 (PubMed).

Gerstner, Bremer, Vander Heyden et al.: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, 2008 (PubMed).

Henson, Roberts, Salimi et al.: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, 2008 (PubMed).

Kiepe, Van Der Pas, Ciarmatori et al.: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, 2008 (PubMed).

Happold, Roth, Wick et al.: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, 2013 (PubMed).

General Burke, Enghild, Martin et al.: "Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH." in: Nature medicine, Vol. 2, Issue 3, pp. 347-50, 1996 (PubMed).

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