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Details for Product No. ABIN185240

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody

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Antigen
Synonyms
G3PD, GAPD, Gapd, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, G3PDH, CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh, Gapdh-2, Gapdh13F, BEST:GH12586, CG12055,  ... show more
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 6 references available
Catalog no. ABIN185240
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Specificity GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GAPDH
Background Synonyms: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Gene ID 2597
NCBI Accession NP_002037.2
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 35 kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1 kDa according to NP_002037). Recommended concentration: 0.01-0.03 µg/mL.
IHC: In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed. Recommended concentration 0.3 µg/mL (data not shown).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Nguyen, Pace, Koller: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, 2007 (PubMed).

Gerstner, Bremer, Vander Heyden et al.: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, 2008 (PubMed).

Henson, Roberts, Salimi et al.: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, 2008 (PubMed).

Kiepe, Van Der Pas, Ciarmatori et al.: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, 2008 (PubMed).

Happold, Roth, Wick et al.: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, 2013 (PubMed).

Burke, Enghild, Martin et al.: "Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH." in: Nature medicine, Vol. 2, Issue 3, pp. 347-50, 1996 (PubMed).

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts (251), (200), (36), (22), (19)
Reactivities (395), (245), (210), (108), (59), (39), (38), (36), (27), (26), (24), (20), (16), (14), (13), (11), (10), (9), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (446), (237), (140), (81), (79), (59), (50), (37), (17), (13), (7), (6), (5), (5), (4), (2), (2), (1), (1)
Conjugates (30), (24), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1)
Epitopes (81), (34), (11), (10), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
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