Msh Homeobox 1 (MSX1) antibody

Antigen: Msh Homeobox 1 (MSX1)

Synonyms: HOX7, HYD1, STHAG1, msh, Hox7, Hox-7, Hox7.1, AA675338, AI324650

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185250

Additional Information

Alternative name: MSX1

Gene ID: 4487, 17701 (mouse)

Immunogen: TSLPLGVKVEDS-C

Format: Affinity-purified

Description: All names and symbols: MSX1, HOX7, HYD1, msh homeo box homolog 1 (Drosophila). Official Symbol: MSX1. RefSeq number(s): NP_002439. Gene ontology terms: development, nucleus, regulation of transcription, DNA-dependent, skeletal development, transcription factor activity

Specificity: Human, Mouse, Rat 297 aa

Application Details

Application Notes: PPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx. 55-60kDa band in Human Muscle and Heart lysates at 1ug/ml. Further, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 31.4kDa according to Human NP_002439.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Prostate shows staining of nucleoli in a few epithelial cells. Recommended concentration, 2-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Organogenesis, Transcription Factors, Neural Stem Cell marker

Restrictions: For Research Use only

Images

ABIN185250 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Blin-Wakkach, Lezot, Ghoul-Mazgar et al.: "Endogenous Msx1 antisense transcript: in vivo and in vitro evidences, structure, and potential involvement in skeleton development in mammals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 13, pp. 7336-41, 2001 (PubMed).