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RAN Binding Protein 1 (RANBP1) antibody

Details for Product No. ABIN185257
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Antigen
Synonyms MGC64314, zeh1094, zgc:85649, hm:zeh1094, wu:fa96a09, wu:fj39g07, HTF9A, Htf9a
Reactivity
Human
(28), (15), (14), (14), (12), (12), (12), (12), (12)
Host
Goat
(23), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(18), (10), (5), (4), (3), (3), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185257
374.27 $
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Immunogen C-LSVKEETKEDAEEKQ
Specificity Human 201 aa
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name RANBP1
Background All names and symbols: RANBP1, RAN binding protein 1. Official Symbol: RANBP1. RefSeq number(s): NP_002873
Gene ID 5902
Application Notes Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 28-30kDa band observed in Hela and HepG2 lysates (predicted MW of 23kDa according to NP_002873). Recommended for use at 0.1-1µg/ml.
Restrictions For Research Use only
Format Liquid
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-RAN Binding Protein 1 (RANBP1) antibody ABIN185257staining (0.1µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-RAN Binding Protein 1 (RANBP1) antibody (2) ABIN185257 (0.05 µg/mL) staining of HELA lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence.
anti-RAN Binding Protein 1 (RANBP1) antibody (3) ABIN185257 (3.8 µg/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Bischoff, Krebber, Smirnova et al.: "Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1." in: The EMBO journal, Vol. 14, Issue 4, pp. 705-15, 1995 (PubMed).

Validation Images
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