Pirin (PIR) antibody

Antigen: Pirin (PIR)

Synonyms: ATPIRIN1, PIRIN, PRN, PRN1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185265

Additional Information

Alternative name: Pirin

Gene ID: 8544

Immunogen: GSSKKVTLSVLSREC

Description: All names and symbols: Pirin, PIR, pirin (iron-binding nuclear protein). Official Symbol: PIR. RefSeq number(s): NP_003653. Gene ontology terms: nucleus, transcription cofactor activity, transcription from Pol II promoter

Specificity: Human, Mouse 290 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 30-35kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653. Recommended concentration: 0.3 - 1.0 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN185265 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Wendler, Kremmer, Foerster et al.: "Identification of pirin, a novel highly conserved nuclear protein." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8482-9, 1997 (PubMed).