Formiminotransferase Cyclodeaminase (FTCD) (internal) antibody

Antigen: Formiminotransferase Cyclodeaminase (FTCD)

Synonyms: FTCD, wu:fp37b11, Ftcd, MGC63647, ftcd, MGC89380, LCHC1, MGC64458

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185268

Additional Information

Alternative name: 58KGolgi protein(Internal)/FTCD

Gene ID: 10841

Immunogen: Peptide with sequence CLREQGRGKDQPGRL, from the internal region of the protein sequence according to NP_006648.1, NP_996848.1

Format: Affinity-purified

Description: All names and symbols: FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase, LC1 autoantigen. Official Symbol: FTCD. RefSeq number(s): NP_006648.1, NP_996848.1. Gene ontology terms: cytoplasm, folic acid and derivative metabolism, folic acid binding, formimidoyltetrahydrofolate cyclodeaminase activity, glutamate formimidoyltransferase activity, histidine metabolism, lyase activity, metabolism, transferase activity Variants (NP_006648.1, NP_996848.1) encode the same protein..

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Pig

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: A 55-60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648 and NP_996848). Recommended concentration: 0.03-0.1µg/ml. Immunohistochemistry: In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Bloom, Brashear: "A novel 58-kDa protein associates with the Golgi apparatus and microtubules." in: The Journal of biological chemistry, Vol. 264, Issue 27, pp. 16083-92, 1989 (PubMed).