SAM Domain, SH3 Domain and Nuclear Localization Signals, 1 (SAMSN1) antibody

Antigen: SAM Domain, SH3 Domain and Nuclear Localization Signals, 1 (SAMSN1)

Synonyms: HACS1, NASH1, SASH2, SH3D6B, Hacs1, 930571B16Rik, 4930571B16Rik, Nash, samsn1, wu:fa92h05, wu:fq83f12, SAMSN1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185276

Additional Information

Alternative name: SAMSN1

Gene ID: 64092

Immunogen: IRLDTLSASLGRSC

Format: Affinity-purified

Description: All names and symbols: SAMSN1, HACS1, SAM domain, SH3 domain and nuclear localisation signals, 1, SH3-SAM adaptor protein, hematopoietic adaptor containing SH3 and SAM domains 1. Official Symbol: SAMSN1. RefSeq number(s): NP_071419.2

Specificity: Please note that the immunogen sequence is present in NP_071419.2 (and in AAK07746) but is no longer present in the latest reference sequence (NP_071419.3 at 05/10/05). This latest version has not been subject to final review by NCBI. This antibody may be recognising one particular isoform. Human 441 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 48kDa band observed in Jurkat Tcell lysate (calculated MW of 41.7kDa according to NP_071419.2). The observed size is consistent with observations with competing products. Recommended concentration: 0.5-1.5µg/ml

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Stem Cells, Hematopoietic Progenitors

Restrictions: For Research Use only

Images

ABIN185276 (1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Zhu, Benn, Li et al.: "The SH3-SAM adaptor HACS1 is up-regulated in B cell activation signaling cascades." in: The Journal of experimental medicine, Vol. 200, Issue 6, pp. 737-47, 2004 (PubMed).