Acetyl LDL Receptor (SCARF1) antibody

Antigen: Acetyl LDL Receptor (SCARF1)

Synonyms: SREC, KIAA0149, MGC47738

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185285

Additional Information

Alternative name: SCARF1 / SREC

Gene ID: 8578

Immunogen: SELDPKGQHVC

Format: Affinity-purified

Description: All names and symbols: SCARF1, SREC, KIAA0149, MGC47738, scavenger receptor class F, member 1, acetyl LDL receptor, scavenger receptor expressed by endothelial cells. Official Symbol: SCARF1. RefSeq number(s): NP_003684, NP_663324, NP_663325, NP_663326, NP_663327. Gene ontology terms: cell adhesion, integral to membrane, low-density lipoprotein binding, low-density lipoprotein catabolism, protein binding, receptor mediated endocytosis, structural molecule activity, transmembrane receptor activity

Specificity: This antibody is expected to recognise all 5 isoforms of SCARF1. Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 / SREC1 protein. Human 830 aa, 342 aa,369 aa, 33 aa, 744 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 85-90kDa band observed in Human Heart lysates (calculated MW of 87.4kDa according to NP_003684,). Recommended concentration: 0.3-1.0µg/ml. An additional faint band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185285 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Adachi, Tsujimoto, Arai et al.: "Expression cloning of a novel scavenger receptor from human endothelial cells." in: The Journal of biological chemistry, Vol. 272, Issue 50, pp. 31217-20, 1998 (PubMed).

Berwin, Delneste, Lovingood et al.: "SREC-I, a type F scavenger receptor, is an endocytic receptor for calreticulin." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 51250-7, 2004 (PubMed).