EH-Domain Containing 2 (EHD2) antibody

Antigen: EH-Domain Containing 2 (EHD2)

Synonyms: PAST2, FLJ96617, BC027084, MGC25606, MGC38650, C130052H20Rik, fj66d02, zgc:77671, wu:fj66d02, MGEPS, MGC64266, EHD2, MGC129001, MGC107790

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185287

Additional Information

Alternative name: EHD2

Gene ID: 30846

Swiss-Prot: NP_055416.2

Immunogen: Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)

Format: Liquid

Description: This antibody is expected to recognise EHD1 protein as well as EHD2. Principal Names: FLJ96617, EH domain containing 2, EH-domain containing 2, PAST2, EHD2. Official Symbol: EHD2

Specificity: This antibody is expected to recognise EHD1 protein as well as EHD2. Human, Mouse, Rat, Zebrafish 543 aa

Molecular Weight: 61.2kDa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 65kDa band observed in Human Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approx. 62kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins

Restrictions: For Research Use only

Images

ABIN185287 staining (0.003µg/ml) of human heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Pohl, Smith, Tachibana et al.: "EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins." in: Genomics, Vol. 63, Issue 2, pp. 255-62, 2000 (PubMed).

Guilherme, Soriano, Furcinitti et al.: "Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 40062-75, 2004 (PubMed).