Retinoid X Receptor, beta (RXRB) antibody

Antigen: Retinoid X Receptor, beta (RXRB)

Synonyms: NR2B2, DAUDI6, RCoR-1, MGC1831, H-2RIIBP, rxrb, RXRB, nr2b2, daudi6, rcor-1, RXRbeta, h-2riibp

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185296

Additional Information

Alternative name: RXR beta

Gene ID: 6257

Immunogen: CRDGMGDSGRDSRSP

Format: Affinity-purified

Description: All names and symbols: RXRB, NR2B2, DAUDI6, RCoR-1, MGC1831, H-2RIIBP, retinoid X receptor, beta, MHC class I promoter binding protein. Official Symbol: RXRB. RefSeq number(s): NP_068811. Gene ontology terms: nucleus, regulation of transcription, DNA-dependent, retinoid-X receptor activity, steroid binding, steroid hormone receptor activity, transcription coactivator activity, transcription factor activity

Specificity: This antibody is expected to recognise an epitope corresponding to aa 70-83 of human RXR beta protein and does not cross-react with either RXR alpha or gamma. Human, Mouse, Rat 533 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 55kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 56.9kDa according to NP_068811). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185296 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Samad, Krezel, Chambon et al.: "Regulation of dopaminergic pathways by retinoids: activation of the D2 receptor promoter by members of the retinoic acid receptor-retinoid X receptor family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 26, pp. 14349-54, 1998 (PubMed).

Krezel, Ghyselinck, Samad et al.: "Impaired locomotion and dopamine signaling in retinoid receptor mutant mice." in: Science (New York, N.Y.), Vol. 279, Issue 5352, pp. 863-7, 1998 (PubMed).