PR Domain Containing 4 (PRDM4) antibody

Antigen: PR Domain Containing 4 (PRDM4)

Synonyms: PFM1, MGC45046, SC-1, AW552272, 1700031E19Rik, 2810470D21Rik, prdm4, MGC53711, MGC132367, PRDM4, pfm1, MGC145200, si:dkey-180p18.8, DKFZp469I1314

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185299

Additional Information

Alternative name: PRDM4 / PFM1

Gene ID: 11108

Immunogen: CSAVYSADESLSAHK

Format: Affinity-purified

Description: All names and symbols: PRDM4, PFM1, MGC45046, PR domain containing 4, PR-domain zinc-finger protein PFM1. Official Symbol: PRDM4. RefSeq number(s): NP_036538. Gene ontology terms: DNA binding, RNA polymerase II transcription factor activity, cell proliferation, nucleus, regulation of transcription, DNA-dependent, signal transduction, transcription from Pol II promoter, zinc ion binding

Specificity: Human, Mouse, Rat 801 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in human and mouse brain lysates (calculated MW of 87.9kDa according to NP_036538). Recommended concentration: 0.3-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185299 (0.3µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Chittka, Arevalo, Rodriguez-Guzman et al.: "The p75NTR-interacting protein SC1 inhibits cell cycle progression by transcriptional repression of cyclin E." in: The Journal of cell biology, Vol. 164, Issue 7, pp. 985-96, 2004 (PubMed).