ADAM Metallopeptidase Domain 12 (ADAM12) antibody

Antigen: ADAM Metallopeptidase Domain 12 (ADAM12)

Synonyms: MCMP, MLTN, MLTNA, MCMPMltna, ADAM12, adam12

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185301

Additional Information

Alternative name: ADAM12

Gene ID: 8038

Immunogen: Peptide with sequence AARPLPVSPARALC, from the N Terminus of the protein sequence according to NP_003465.3, NP_067673.2

Format: Affinity-purified

Description: All names and symbols: ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha, A disintegrin and metalloproteinase domain 12 (Meltrin-alpha, mouse, homolog of), ADAM metallopeptidase domain 12, RP11-295J3.5. Official Symbol: ADAM12. RefSeq number(s): NP_003465.3, NP_067673.2. Gene ontology terms: cell adhesion, hydrolase activity, integral to membrane, metalloendopeptidase activity, myoblast fusion, plasma membrane, protein binding, proteolysis and peptidolysis, zinc ion binding This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS..

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 90kDa band observed in human heart lysates (calculated MW of 99.6kDa according to NP_003465 and 80.5kDa according to NP_067673). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasm staining of a group of secretory epithelial cells. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Proteases

Restrictions: For Research Use only

Publications

Publications: Gilpin, Loechel, Mattei et al.: "A novel, secreted form of human ADAM 12 (meltrin alpha) provokes myogenesis in vivo." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 157-66, 1998 (PubMed).

Galliano, Huet, Frygelius et al.: "Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion." in: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13933-9, 2000 (PubMed).

Tanaka, Nanba, Mori et al.: "ADAM binding protein Eve-1 is required for ectodomain shedding of epidermal growth factor receptor ligands." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 41950-9, 2004 (PubMed).