Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) antibody
| Antigen |
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| Synonyms |
ADCAD2, FLJ90062, FLJ90421, Cd, C030016K15Rik, lrp6-A, Xlrp6, lrp6-B, LRP6 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Application
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| Catalog no. |
ABIN185307 |
| Quantity |
100 ug |
| Price |
374.27 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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LRP6
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Gene ID
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4040, 16974 (mouse)
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Immunogen
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CMTSVATAKGYTSDL
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Description
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All names and symbols: LRP6, low density lipoprotein receptor-related protein 6, low density lipoprotein-related protein 6. Official Symbol: LRP6. RefSeq number(s): NP_002327.2. Gene ontology terms: Wnt receptor signaling pathway, development, endocytosis, integral to membrane, plasma membrane, protein binding, receptor activity
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Specificity
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This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein. Human, Mouse, Rat, Chicken 1613 aa
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: experiments gave bands at approx 250kDa, 150kDa, 50kDa and 35kDa in human adipose tissue lysate after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180kDa according to NP_002327. All detected bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded human liver shows strong cytoplasmic staining in hepatocytes and cytoplasmic staining in bile ductulus cells. Recommended concentration, 5-10µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Stem Cells, Wnt
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Restrictions
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For Research Use only
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Tamai, Semenov, Kato et al.: "LDL-receptor-related proteins in Wnt signal transduction." in: Nature, Vol. 407, Issue 6803, pp. 530-5, 2000 (PubMed).
Giles, van Es, Clevers: "Caught up in a Wnt storm: Wnt signaling in cancer." in: Biochimica et biophysica acta, Vol. 1653, Issue 1, pp. 1-24, 2003 (PubMed).
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Alternatives for antigen "Low Density Lipoprotein Receptor-Related Protein 6 (LRP6)", type "Antibodies"
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Hosts
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Rabbit (40), Goat (3)
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Reactivities
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Human (41), Cow (Bovine) (28), Dog (Canine) (28), Horse (Equine) (28), Mouse (Murine) (28), Pig (Porcine) (28), Rat (Rattus) (23), Chicken (5), Guinea Pig (5), Purple Sea Urchin (Strongylocentrotus purpuratus) (1)
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Applications
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Immunofluorescence (IF) (25), Flow Cytometry (FACS) (15), ELISA (12), Western Blotting (WB) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Epitopes
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pSer1490 (5), C-Term (4), pThr1479 (4), AA 1546-1560 (2), C-Term,Thr1546 (1), Internal Region (1), pSer663 (1), pThr1492 (1)
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Alternatives 
2013 BioMed Central Research Awards
