Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) antibody

Antigen: Low Density Lipoprotein Receptor-Related Protein 6 (LRP6)

Synonyms: ADCAD2, FLJ90062, FLJ90421, Cd, C030016K15Rik, lrp6-A, Xlrp6, lrp6-B, LRP6

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185307

Additional Information

Alternative name: LRP6

Gene ID: 4040, 16974 (mouse)

Immunogen: CMTSVATAKGYTSDL

Format: Affinity-purified

Description: All names and symbols: LRP6, low density lipoprotein receptor-related protein 6, low density lipoprotein-related protein 6. Official Symbol: LRP6. RefSeq number(s): NP_002327.2. Gene ontology terms: Wnt receptor signaling pathway, development, endocytosis, integral to membrane, plasma membrane, protein binding, receptor activity

Specificity: This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein. Human, Mouse, Rat, Chicken 1613 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: experiments gave bands at approx 250kDa, 150kDa, 50kDa and 35kDa in human adipose tissue lysate after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180kDa according to NP_002327. All detected bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded human liver shows strong cytoplasmic staining in hepatocytes and cytoplasmic staining in bile ductulus cells. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Stem Cells, Wnt

Restrictions: For Research Use only

Images

ABIN185307 (5µg/ml) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Tamai, Semenov, Kato et al.: "LDL-receptor-related proteins in Wnt signal transduction." in: Nature, Vol. 407, Issue 6803, pp. 530-5, 2000 (PubMed).

Giles, van Es, Clevers: "Caught up in a Wnt storm: Wnt signaling in cancer." in: Biochimica et biophysica acta, Vol. 1653, Issue 1, pp. 1-24, 2003 (PubMed).