Coiled-Coil Domain Containing 50 (CCDC50) antibody

Antigen: Coiled-Coil Domain Containing 50 (CCDC50)

Synonyms: C3orf6, AW048328, AW546090, D16Bwg1543e, 2610529H08Rik, 5730448P06Rik, YMER, DFNA44, c3orf6, C3orf6h, MGC133563, ymer

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185310

Additional Information

Alternative name: Ymer / CCDC50

Gene ID: 152137

Immunogen: CFSKSESSHKGFHYK

Format: Affinity-purified

Description: All names and symbols: coiled-coil domain containing 50, C3orf6, YMER, Ymer protein, C3orf6, chromosome 3 open reading frame 6. Official Symbol: CCDC50. RefSeq number(s): NP_848018.1, NP_777568.1

Specificity: This antibody is expected to recognise both reported isoforms of human Ymer protein. Human, Chimpanzee, Rat 482aa, 306 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 18kDa band in Human Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature fo Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185310 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Blagoev, Ong, Kratchmarova et al.: "Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics." in: Nature biotechnology, Vol. 22, Issue 9, pp. 1139-45, 2004 (PubMed).