Feline Leukemia Virus Subgroup C Cellular Receptor 1 (FLVCR1) antibody

Antigen: Feline Leukemia Virus Subgroup C Cellular Receptor 1 (FLVCR1)

Synonyms: FLVCR, Flvcr1, 9630055N22Rik, FLVCR1, si:ch211-147d7.6

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185315

Additional Information

Alternative name: FLVCR

Gene ID: 28982

Immunogen: C-KTVMLSKQSESAI

Format: Affinity-purified

Description: All names and symbols: FLVCR, feline leukemia virus subgroup C cellular receptor, FLVCR protein. Official Symbol: FLVCR. RefSeq number(s): NP_054772.1. Gene ontology terms: development, integral to plasma membrane, receptor activity, transport, transporter activity

Specificity: Human, Chimpanzee, Cat 555 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 59.9kDa according to NP_054772.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? ABIN185315 has been successfully used in WB application in the publicatioon: Rey M.A. et al. Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis. Haematologica 2008 Nov,93(11):1617-26. Epub 2008 Sep 24. PMID:18815190.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Stem Cells, Hematopoietic Progenitors

Restrictions: For Research Use only

Images

ABIN185315 (0.1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Quigley, Yang, Worthington et al.: "Identification of a human heme exporter that is essential for erythropoiesis." in: Cell, Vol. 118, Issue 6, pp. 757-66, 2004 (PubMed).