Zinc Finger, DHHC-Type Containing 17 (ZDHHC17) antibody

Antigen: Zinc Finger, DHHC-Type Containing 17 (ZDHHC17)

Synonyms: HIP3, HYPH, HIP14, HSPC294, KIAA0946, si:ch211-81a6.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185335

Additional Information

Alternative name: HIP14 / ZDHHC17

Gene ID: 23390

Immunogen: CYDQISGSGYQLV

Format: Affinity-purified

Description: All names and symbols: ZDHHC17, HIP14, HIP3, HYPH, KIAA0946, zinc finger, DHHC domain containing 17, Huntingtin interacting protein H, huntingtin interacting protein 3, huntingtin interacting protein 14. Official Symbol: ZDHHC17. RefSeq number(s): NP_056151.1. Gene ontology terms: Golgi apparatus, integral to membrane, metal ion binding, positive regulation of I-kappaB kinase/NF-kappaB cascade, signal transducer activity

Specificity: Human, Dog, Mouse, Rat, Chimpanzee 633 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 70kDa band observed in Human and Mouse Brain lysates (calculated MW of 72.7kDa according to NP_056151.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185335 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Huang, Yanai, Kang et al.: "Huntingtin-interacting protein HIP14 is a palmitoyl transferase involved in palmitoylation and trafficking of multiple neuronal proteins." in: Neuron, Vol. 44, Issue 6, pp. 977-86, 2004 (PubMed).