CD274 Molecule (CD274) antibody

Antigen: CD274 Molecule (CD274)

Synonyms: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, MGC142294, MGC142296, B7h1, Pdl1, Pdcd1l1, Pdcd1lg1, A530045L16Rik, CD274

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185339

Additional Information

Alternative name: CD274 / PD-L1

Gene ID: 29126

Immunogen: CKKQSDTHLEET

Description: All names and symbols: CD274 antigen, PD-L1, PDCD1LG1, B7-H, B7H1, PDL1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635. Official Symbol: CD274. RefSeq number(s): NP_054862.1. Gene ontology terms: cell proliferation, cell surface receptor linked signal transduction, immune response, integral to membrane

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35+37kDa double band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). Recommended concentration: 0.3-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185339 staining (0.3µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Thompson, Gillett, Cheville et al.: "Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 49, pp. 17174-9, 2004 (PubMed).