AKR1C3 antibody (C-Term)

Catalog No. ABIN185345

The Goat Polyclonal anti-AKR1C3 antibody is suitable to detect AKR1C3 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for AKR1C3 antibody (C-Term) (ABIN185345)

Target: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKR1C3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-AKR1C3 Antibody

Binding Specificity: C-Term

Purpose: AKR1C3

Sequence: CFASHPNYPY SDEY

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CFASHPNYPYSDEY, from the C Terminus of the protein sequence according to NP_003730.4.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in Human Liver and Human Breast lysates (calculated MW of 36.9 kDa according to NP_003730). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AKR1C3

Target: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Alternative Name: AKR1C3

Background: AKR1C3, DD3, HAKRB, HAKRe, HA1753, HSD17B5, hluPGFS, KIAA0119, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II), prostaglandin F synthase, dihydrodiol dehydrogenase 3, chlordecone reductase homolog, hydroxysteroid (1

Gene ID: 8644

NCBI Accession: NP_003730

Pathways: Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Protein targeting to Nucleus