Triosephosphate Isomerase 1 (TPI1) antibody

Antigen: Triosephosphate Isomerase 1 (TPI1)

Synonyms: TPI1, TIM, tpi, TPI, DKFZp459E144, tpi1, MGC88108, Tpi, Tpi-1, AI255506, MGC156639, MGC52638, GB17473

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185349

Additional Information

Alternative name: Triosephosphate isomerase

Gene ID: 7167

Immunogen: Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1, NP_001152759.1

Format: Affinity-purified

Description: All names and symbols: TPI1, triosephosphate isomerase 1, TPI, MGC88108. Official Symbol: TPI1. RefSeq number(s): NP_000356.1, NP_001152759.1. Gene ontology terms: fatty acid biosynthesis, gluconeogenesis, glycolysis, isomerase activity, metabolism, pentose-phosphate shunt, triose-phosphate isomerase activity This antibody is expected to recognise both reported isoforms..

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 26kDa band observed in human liver and human hepatoblastoma HepG2 cell lysates (calculated MW of 26.7kDa according to NP_000356). Recommended concentration: 0.01-0.03µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Cancer, Metabolism

Restrictions: For Research Use only

Publications

Publications: Jiang, Gan, Huang et al.: "Proteomics-based identification of proteins with altered expression induced by 12-O-tetradecanoylphorbol 13-acetate in nasopharyngeal carcinoma CNE2 cells." in: Acta biochimica et biophysica Sinica, Vol. 37, Issue 2, pp. 97-106, 2005 (PubMed).