Asporin (ASPN) antibody

Antigen: Asporin (ASPN)

Synonyms: OS3, PLAP1, SLRR1C, FLJ20129, bgl3, aspnl, wu:fk08c11, zgc:109936, Plap1, Slrr1c, AA986886, 4631401G09Rik, MGC128677

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN185364

Additional Information

Alternative name: Asporin / ASPN

Gene ID: 54829

Immunogen: C-IHENKVKKIQKDT

Format: Affinity-purified

Description: All names and symbols: ASPN, PLAP1, SLRR1C, FLJ20129, asporin (LRR class 1), small leucine-rich protein 1C, periodontal ligament associated protein 1. Official Symbol: ASPN. RefSeq number(s): NP_060150.3. Gene ontology terms: extracellular matrix (sensu Metazoa)

Specificity: Human, Mouse, Rat 381 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 40kDa band observed in Human Bone Marrow lysates (calculated MW of 43.4kDa according to NP_060150.3). Recommended concentration: 1-3µg/ml. Immunofluoresence in cell line ATDC5 shows signals in cytoplasm and on the cell surface. Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185364 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185364 staining of ATDC5 cells (Panels A and C) and DAPI (panels B and D). Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.

Publications

Publications: Kizawa, Kou, Iida et al.: "An aspartic acid repeat polymorphism in asporin inhibits chondrogenesis and increases susceptibility to osteoarthritis." in: Nature genetics, Vol. 37, Issue 2, pp. 138-44, 2005 (PubMed).