Retinoic Acid Receptor, alpha (RARA) antibody

Antigen: Retinoic Acid Receptor, alpha (RARA)

Synonyms: RAR, NR1B1, Nr1b1, RARalpha1, RARA, rar-alpha

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185366

Additional Information

Alternative name: RARA

Gene ID: 5914

Immunogen: C-SPSLSPSSHRSSPATQSP

Format: Affinity-purified

Description: All names and symbols: RARA, RAR, NR1B1, retinoic acid receptor, alpha, Retinoic acid receptor, alpha polypeptide, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR short form. Official Symbol: RARA. RefSeq number(s): NP_000955.1, NP_001019980.1, NP_001028775.1. Gene ontology terms: nucleus, regulation of transcription, DNA-dependent, retinoic acid receptor activity, signal transduction, steroid hormone receptor activity, transcription coactivator activity, transcription factor activity

Specificity: NP_000955.1 (isfa) and NP_001028775.1(alpha) represent identical proteins. This antibody is expected to recognize all three reported isoforms (NP_000955.1, NP_001019980.1, NP_001028775.1). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 50+48kDa bands observed in Human Brain lysates (calculated MW of 50.8kDa according to NP_000955.1, NP_001019980.1 and NP_001028775.1). Recommended concentration: 0.03-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185366 staining (0.03µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Farias, Ong, Ghyselinck et al.: "Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity." in: Journal of the National Cancer Institute, Vol. 97, Issue 1, pp. 21-9, 2005 (PubMed).