SERPINI2 antibody (Internal Region)

Catalog No. ABIN185374

This Goat Polyclonal antibody specifically detects SERPINI2 in WB, ELISA and IHC. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for SERPINI2 antibody (Internal Region) (ABIN185374)

Target: SERPINI2 (serpin Peptidase Inhibitor, Clade I (Pancpin), Member 2 (SERPINI2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SERPINI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SERPINI2 Antibody

Binding Specificity: Internal Region

Purpose: MEPI / SERPINI2

Sequence: KGDWKQKFRK EDTQ

Specificity: This antibody is expected to recognise both reported isoforms (NP_006208.1, NP_001012303.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KGDWKQKFRKEDTQ, from the internal region of the protein sequence according to NP_006208.1, NP_001012303.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cels and of rare cells in the islets of Langerhans. Recommended concentration, 3 μg/mL. This product has been successfully used in IHC on Human in PMID: 214992

Western Blot: Approx 40 kDa band observed in Human Pancreas lysates (calculated MW of 46.1 kDa according to NP_006208.1 and 47.4 kDa according to NP_001012303.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. This product has been s

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SERPINI2 antibody (ABIN185374)

Wehr, Furth, Sangar, Blair, Yu: "Analysis of the human pancreatic stellate cell secreted proteome." in: Pancreas, Vol. 40, Issue 4, pp. 557-66, (2011) (PubMed).

Target Details for SERPINI2

Target: SERPINI2 (serpin Peptidase Inhibitor, Clade I (Pancpin), Member 2 (SERPINI2))

Alternative Name: SERPINI2

Background: SERPINI2, MEPI, PI14, PANCPIN, TSA2004, SERPIN12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2, serpin peptidase inhibitor, clade I (pancpin), member 2, myoepithelium-derived serine protease inhibitor, protease inhibitor 14,

Gene ID: 5276, 67931, 171149

NCBI Accession: NP_006208, NP_001012303