Apolipoprotein E (APOE) antibody

Antigen: Apolipoprotein E (APOE)

Synonyms: AD2, LPG, LDLCQ5, MGC1571, AI255918, APOEA, APOE, ad2, apoprotein, MGC110064, im:7036787, wu:fb69a05, zgc:110064, apoe

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185375

Additional Information

Alternative name: APOE

Gene ID: 348

Immunogen: C-VGTSAAPVPSDNH

Format: Affinity-purified

Description: All names and symbols: APOE, AD2, MGC1571, apoprotein, apolipoprotein E, apolipoprotein E3, Alzheimer disease 2 (APOE*E4-associated, late onset). Official Symbol: APOE. RefSeq number(s): NP_000032.1. Gene ontology terms: antioxidant activity, apolipoprotein E receptor binding, beta-amyloid binding, cholesterol homeostasis, circulation, cytoplasm, cytoskeleton organization and biogenesis, extracellular region, extracellular region, heparin binding, induction of apoptosis, intracellular transport, learning and/or memory, lipid binding, lipid transport, lipid transporter activity, lipoprotein metabolism, regulation of axon extension, regulation of neuronal synaptic plasticity, response to reactive oxygen species, synaptic transmission, cholinergic, tau protein binding

Specificity: Human, Chimpanzee 317 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 36kDa band observed in Human Brain lysates (calculated MW of 36.2kDa according to NP_000032.1). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded Human Liver shows extra-cellular staining between hepatocytes. Recommended concentration, 2-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185375 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185375 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Dodart, Marr, Koistinaho et al.: "Gene delivery of human apolipoprotein E alters brain Abeta burden in a mouse model of Alzheimer's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 4, pp. 1211-6, 2005 (PubMed).

Levi, Jongen-Relo, Feldon et al.: "Brain area- and isoform-specific inhibition of synaptic plasticity by apoE4." in: Journal of the neurological sciences, Vol. 229-230, pp. 241-8, 2005 (PubMed).