Melanocortin 3 Receptor (MC3R) antibody

Antigen: Melanocortin 3 Receptor (MC3R)

Synonyms: MC3, OB20, OQTL, BMIQ9, MC3-R, MGC124211, MGC124212, zgc:194235, MC3R

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185386

Additional Information

Alternative name: Melanocortin 3 Receptor

Gene ID: 4159

Immunogen: SIQKTYLEGDFV-C

Format: Affinity-purified

Description: All names and symbols: melanocortin 3 receptor, MC3R, MC3. Official Symbol: MC3R. RefSeq number(s): NP_063941.2. Gene ontology terms: G-protein signaling, coupled to IP3 second messenger (phospholipase C activating), G-protein signaling, coupled to cyclic nucleotide second messenger, integral to plasma membrane, melanocortin receptor activity, rhodopsin-like receptor activity

Specificity: Human 360 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 40kDa band observed in Human Brain lysates (calculated MW of 40.1kDa according to NP_063941.2). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185386 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Muehlhaeusler, Adam, Marrocco et al.: "Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation." in: The Journal of physiology, Vol. 565, Issue Pt 1, pp. 185-95, 2005 (PubMed).