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Proopiomelanocortin (POMC) antibody

Antigen

Proopiomelanocortin (POMC)

Synonyms MSH, ACTH, pomc, SI:bZ36D5.1, SI:bZ36D5.4, si:bz36d5.2, POMC, LPH, NPP, POC, CLIP
Clonality Polyclonal
Host
Reactivity
Alternatives

Human, Mouse (Murine)

Application
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
1 reference available
Catalog no. ABIN185387
Quantity 100µg  (100µg/200µl)  (Variants)
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Alternative name Proopiomelanocortin / POMC
Gene ID 5443
Swiss-Prot NP_000930.1
Immunogen Peptide with sequence C-NAIIKNAYKKGE, from the C Terminus of the protein sequence according to NP_000930.1, NP_001030333.1.
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Format Liquid
Grade Serum-free
Description Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only. Official Symbol: POMC
Specificity Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only. Human, Mouse, Rat 267 aa

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx. 26kDa band observed in lysates of Mouse cell line NIH3T3 ((calculated MW of 29.4kDa according to Human NP_000930.1 and of 26.7kDa according to Mouse NP_032921.1). Recommended concentration: 1-3µg/ml. IHC: In paraffin embedded Human Adrenal Gland shows strong cytoplasm staining in select cells in the medulla. Recommended concentration, 2-4µg/ml. Data obtained from previous goat (showing identical WB data).
Concentration 100µg/200µl
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Publications Muehlhaeusler, Adam, Marrocco et al.: "Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation." in: The Journal of physiology, Vol. 565, Issue Pt 1, pp. 185-95, 2005 (PubMed).