Cathepsin F (CTSF) antibody

Antigen: Cathepsin F (CTSF)

Synonyms: CATSF, AI481912, MGC124702, CTSF, MGC140519, cb420, sb:cb420, MGC153093, zgc:153093, LOC100101596, Ctsf, ACYPI000376, catf

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185391

Additional Information

Alternative name: Cathepsin F

Gene ID: 8722

Immunogen: CGVNTMASSAVVD

Description: All names and symbols: CTSF, cathepsin F , HGNC:2531, CATSF. Official Symbol: CTSF. RefSeq number(s): NP_003784.2

Specificity: Human, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approx 30kDa and 35kDa in Human Prostate and Ovary lysates after 0.3µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Wang et al, J Biol Chem. 1998 Nov 27,273(48):32000-8, PMID: 9822672). This protein has a calculated MW of 53.4kDa according to NP_003784.2. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Immunology, Innate Immunity, Proteases

Restrictions: For Research Use only

Images

ABIN185391 (0.3µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Dorn, Zhao, Granberg et al.: "Identification of specific cellular genes up-regulated late in adenovirus type 12 infection." in: Journal of virology, Vol. 79, Issue 4, pp. 2404-12, 2005 (PubMed).