Arrestin 3 antibody (Internal Region)

Catalog No. ABIN185395

This Goat Polyclonal antibody specifically detects Arrestin 3 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for Arrestin 3 antibody (Internal Region) (ABIN185395)

Target: Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Arrestin 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Arrestin 3 Antibody

Binding Specificity: Internal Region

Purpose: Arrestin beta 2

Sequence: HDHIPLPRPQ S

Specificity: This antibody is expected to recognise reported isoforms 1, 2, 3, 5, 6 and 7. No crossreactivity is expected with Arrestin beta 1.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1, NP_945355.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in lysates of cell lines A549 and U251 and in preliminary testing of KNRK cell lysate (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 kDa according to NP_945355.1) Recommended concentration: 0.5-1 μg/mL

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Arrestin 3 antibody (ABIN185395)

Zakrzewicz, Krasteva, Wilhelm, Dietrich, Wilker, Padberg, Wygrecka, Grau: "Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys." in: Immunobiology, Vol. 216, Issue 7, pp. 854-61, (2011) (PubMed).

Napolitano, Pasqualetti, Usiello, Santini, Pacini, Sciamanna, Errico, Tassone, Di Dato, Martella, Cuomo, Fisone, Bernardi, Mandolesi, Mercuri, Standaert, Pisani: "Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism in a model of DYT1 dystonia." in: Neurobiology of disease, Vol. 38, Issue 3, pp. 434-45, (2010) (PubMed).

Target Details for Arrestin 3

Target: Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

Alternative Name: ARRB2

Background: ARRB2, arrestin, beta 2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2, BARR2, arrestin 3

Molecular Weight: 46.1kDa

Gene ID: 409, 216869, 25388

NCBI Accession: NP_004304, NP_945355, NP_001244257, NP_001244259, NP_001244260

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Phototransduction, Thromboxane A2 Receptor Signaling