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Arrestin, beta 2 (ARRB2) antibody
| Antigen | Arrestin, beta 2 (ARRB2) |
| Synonyms | ARB2, ARR2, BARR2, DKFZp686L0365, BARRES, MGC105502, AI326910, AW122872, RP23-42P20.7, ARRB2, arrb2, MGC64007, zgc:64007, arb2, arr2, barr2, MGC82523, arrestin, betaarr2, DKFZp468L2113 |
| Clonality | Polyclonal |
| Host |
Alternatives Goat |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives ELISA, Western Blotting (WB), Immunoprecipitation (IP)
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1 reference available |
| Catalog no. | ABIN185395 |
| Quantity | 100 µg (100µg/200µl) (Variants) |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | Arrestin beta 2 |
| Gene ID | 409 |
| Swiss-Prot | NP_004304.1 |
| Immunogen | Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1, NP_945355.1. |
| Cross-Reactivity | Human, Mouse (Murine), Rat (Rattus) |
| Format | Liquid |
| Description | This antibody is expected to recognise both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1. Principal Names: BARR2, beta-arrestin 2, arrestin beta 2, DKFZp686L0365, ARR2, ARB2, HGNC:712, ARRB2, arrestin, beta 2. Official Symbol: ARRB2 |
| Specificity | This antibody is expected to recognise both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1. Human, Mouse, Rat 409 aa, 394 aa |
| Molecular Weight | 46.1kDa |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 40kDa band observed in Human Brain lysates (calculated MW of 46.1kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1µg/ml. IHC: In paraffin embedded Human Cerebellum shows mainly nuclear staining of purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6µg/ml. Immunoprecipitation: This antibody has been successfully used in IP from rat leukocytes as described in the following paper: Zakrzewicz et al, Immunobiology. 2011 Jul,216(7):854-61, PMID: 21193245. |
| Concentration | 100µg/200µl |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN185395 (0.03µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publications
| Publications |
Lefkowitz, Shenoy: "Transduction of receptor signals by beta-arrestins." in: Science (New York, N.Y.), Vol. 308, Issue 5721, pp. 512-7, 2005 (PubMed).
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Alternatives
Alternatives for antigen "Arrestin, beta 2 (ARRB2)", type "Antibodies"
| Hosts | Mouse (6), Goat (4), Rabbit (3) |
| Reactivities | Human (13), Rat (Rattus) (5), Mouse (Murine) (4), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1), Monkey (1) |
| Applications | Western Blotting (WB) (13), Enzyme Immunoassay (EIA) (6), Immunofluorescence (IF) (4), ELISA (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (2), ELISA (Detection) (1) |




Alternatives