Arrestin, beta 2 (ARRB2) antibody

Antigen: Arrestin, beta 2 (ARRB2)

Synonyms: ARB2, ARR2, BARR2, DKFZp686L0365, BARRES, MGC105502, AI326910, AW122872, RP23-42P20.7, ARRB2, arrb2, MGC64007, zgc:64007, arb2, arr2, barr2, MGC82523, arrestin, betaarr2, DKFZp468L2113

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN185395

Additional Information

Alternative name: Arrestin beta 2

Gene ID: 409

Swiss-Prot: NP_004304.1

Immunogen: Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1, NP_945355.1.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Format: Liquid

Description: This antibody is expected to recognise both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1. Principal Names: BARR2, beta-arrestin 2, arrestin beta 2, DKFZp686L0365, ARR2, ARB2, HGNC:712, ARRB2, arrestin, beta 2. Official Symbol: ARRB2

Specificity: This antibody is expected to recognise both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1. Human, Mouse, Rat 409 aa, 394 aa

Molecular Weight: 46.1kDa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 40kDa band observed in Human Brain lysates (calculated MW of 46.1kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1µg/ml. IHC: In paraffin embedded Human Cerebellum shows mainly nuclear staining of purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6µg/ml. Immunoprecipitation: This antibody has been successfully used in IP from rat leukocytes as described in the following paper: Zakrzewicz et al, Immunobiology. 2011 Jul,216(7):854-61, PMID: 21193245.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185395 (0.03µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Lefkowitz, Shenoy: "Transduction of receptor signals by beta-arrestins." in: Science (New York, N.Y.), Vol. 308, Issue 5721, pp. 512-7, 2005 (PubMed).