Vacuolar Protein Sorting 37 Homolog C (S. Cerevisiae) (VPS37C) antibody

Antigen: Vacuolar Protein Sorting 37 Homolog C (S. Cerevisiae) (VPS37C)

Synonyms: FLJ20847, AU042646, 5730409F24Rik, RGD1309258, DKFZp468H155

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185432

Additional Information

Alternative name: VPS37C

Gene ID: 55048

Immunogen: C-ETLKDKTLQELEELQ

Description: All names and symbols: VPS37C, vacuolar protein sorting 37C (yeast) , FLJ20847 , vacuolar protein sorting 37C. Official Symbol: VPS37C. RefSeq number(s): NP_060436.4

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 38kDa band observed in human duodenum lysates (calculated MW of 38.7kDa according to NP_060436.4). Recommended concentration: 0.03 - 0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185432 (0.03g/ml) staining of human duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Eastman, Martin-Serrano, Chung et al.: "Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding." in: The Journal of biological chemistry, Vol. 280, Issue 1, pp. 628-36, 2004 (PubMed).