Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody

Antigen: Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1)

Synonyms: KLR, CD314, NKG2D, NKG2-D, FLJ17759, FLJ75772, D12S2489E, KLRK1, Nkrp2, Nkg2d, D6H12S2489E

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185435

Additional Information

Alternative name: NKG2D / KLRK1

Gene ID: 22914

Immunogen: C-KVYSKEDQDLLK

Format: Affinity-purified

Description: All names and symbols: KLRK1, NKG2-D, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NK cell receptor D, NKG2-D type II integral membrane protein. Official Symbol: KLRK1. RefSeq number(s): NP_031386.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in human lymph node and human spleen lysates (calculated MW of 25.3kDa according to NP_031386.1). The observed molecular weight is explained by glycosylation (Han et al, Blood. 2004 Nov 1,104(9):2858-66. Epub 2004 Jul 6, PMID: 15238421). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185435 staining (0.3µg/ml) of human spleen lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Blower, Nachury, Heald et al.: "A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly." in: Cell, Vol. 121, Issue 2, pp. 223-34, 2005 (PubMed).

Hayakawa, Smyth: "Innate immune recognition and suppression of tumors." in: Advances in cancer research, Vol. 95, pp. 293-322, 2006 (PubMed).