Complement Factor H (CFH) antibody

Antigen: Complement Factor H (CFH)

Synonyms: FH, HF, HF1, HF2, HUS, FHL1, AHUS1, ARMD4, ARMS1, CFHL3, MGC88246, NOM, Sas1, Mud-1, Sas-1, Fh, CFH, MGC129039, im:7147412, si:ch211-207o17.2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185437

Additional Information

Alternative name: Complement factor H

Gene ID: 3075

Immunogen: Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2

Description: Goat anti-Complement factor H antibody. Principal Names: complement factor H ; HGNC:4883; CFH; CFHL3; FHL1; HF; HF1; HF2; HUS ; H factor 1 (complement); H factor 2 (complement); H factor-1 (complement); factor H-like 1. Official Gene Symbol: CFH. Accession Number(s): NP_000177.2. Human Gene ID(s): 3075. This antibody is expected to recognize isoform a (NP_000177.2) only.

Specificity: This antibody is expected to recognize isoform a (NP_000177.2) only. Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 170kDa band observed in human lung lysates (calculated MW of 139kDa according to NP_000177.2). Recommended concentration: 0.03-0.1μg/ml.

Concentration: 0.5 mg/ml

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Complement Factors

Restrictions: For Research Use only

Images

ABIN185437 staining (0.03µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Klein, Zeiss, Chew et al.: "Complement factor H polymorphism in age-related macular degeneration." in: Science (New York, N.Y.), Vol. 308, Issue 5720, pp. 385-9, 2005 (PubMed).