»
Antibodies
»
anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU Class 5 Homeobox 1 (POU5F1) antibody
| Antigen |
|
| Synonyms |
OCT3, OCT4, OTF3, OTF4, MGC22487, POU5F1, POU5F1L |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
|
| Catalog no. |
ABIN185438 |
| Quantity |
100 ug (Variants) |
| Price |
501.80 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
OCT4 / POU5F1
|
|
Gene ID
|
5460, 18999 (mouse), 294562 (rat)
|
|
Immunogen
|
C-VTTLGSPMHSN
|
|
Format
|
Affinity-purified
|
|
Description
|
All names and symbols: POU5F1, OTF4, POU domain, class 5, transcription factor 1, HGNC:9221, MGC22487, OCT3, OTF3, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3. Official Symbol: POU5F1. RefSeq number(s): NP_002692.2, NP_976034.2
|
|
Specificity
|
This antibody is expected to recognise both reported isoforms (NP_002692 and NP_976034) Human, Mouse, Rat, Dog
|
|
Application Notes
|
Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 38kDa band observed in Human Liver lysates (calculated MW of 38.6kDa according to NP_002692.2). Recommended concentration: 1-3µg/ml.
|
|
Purification
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Buffer
|
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|
|
Storage
|
Aliquot and store at -20°C. Minimize freezing and thawing.
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Jones, Ulbright, Eble et al.: "OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma." in: The American journal of surgical pathology, Vol. 28, Issue 7, pp. 935-40, 2004 (PubMed).
Chew, Loh, Zhang et al.: "Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells." in: Molecular and cellular biology, Vol. 25, Issue 14, pp. 6031-46, 2005 (PubMed).
|
Alternatives for antigen "POU Class 5 Homeobox 1 (POU5F1)", type "Antibodies"
|
Hosts
|
Rabbit (42), Mouse (16), Goat (6)
|
|
Reactivities
|
Human (50), Mouse (Murine) (15), Rat (Rattus) (9), Cow (Bovine) (3), Dog (Canine) (3), Chimpanzee (2), Horse (Equine) (2), Monkey (2), Pig (Porcine) (1), Primate (1)
|
|
Applications
|
Western Blotting (WB) (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Enzyme Immunoassay (EIA) (18), ELISA (10), Immunofluorescence (IF) (5), Immunoprecipitation (IP) (5), Immunohistochemistry (IHC) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1)
|
|
Epitopes
|
N-Term (2), AA 100-200 (1), AA 230-244 (1), E125 (1), transcript variant 1 (1)
|
Alternatives 
