Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (C-Term) antibody

Details for Product No. ABIN185447
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Antigen
Synonyms 0710008N11Rik, Ip, succinate dehydrogenase 2-3, CG3283, Dmel\\CG3283, SDH, SDH-IP, SDH-Ip, SDHb, sdhB, CWS2, IP, PGL4, SDH1, SDH2, SDHIP, fj46d06, wu:fj46d06
Epitope
C-Term
(8), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(41), (27), (18), (13), (13), (13), (1), (1), (1), (1), (1)
Host
Goat
(39), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(35), (13), (10), (10), (10), (6), (5), (2), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN185447
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
Options
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Availability Available

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  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SDHB
Background Synonyms: SDHB, IP, SDH, SDH1, SDHIP, succinate dehydrogenase complex, subunit B, iron sulfur (Ip), OTTHUMP00000044624, iron-sulfur subunit, FLJ92337, SDH2, SDHIP
Gene ID 6390
NCBI Accession NP_002991.2
Research Area Metabolism
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 28 kDa band observed in Human Liver lysates (calculated MW of 31.6 kDa according to NP_002991.1). Recommended concentration: 0.03-0.1 µg/mL. ELISA: Customer successfully used this product in quantitative ELISA on HepG2 lysates.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (C-Term) antibody ABIN185447 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Morris, Maina, Morgan et al.: "Molecular genetic analysis of FIH-1, FH, and SDHB candidate tumour suppressor genes in renal cell carcinoma." in: Journal of clinical pathology, Vol. 57, Issue 7, pp. 706-11, 2004 (PubMed).

Selak, Armour, MacKenzie et al.: "Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase." in: Cancer cell, Vol. 7, Issue 1, pp. 77-85, 2005 (PubMed).

Hosts (39), (6)
Reactivities (41), (27), (18), (13), (13), (13), (1), (1), (1), (1), (1)
Applications (35), (13), (10), (10), (10), (6), (5), (2), (1), (1), (1)
Conjugates (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1)
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