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Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (C-Term) antibody

Details for Product No. ABIN185447, Supplier: Login to see
Antigen
  • succinate dehydrogenase 2-3
  • CG3283
  • Dmel\\CG3283
  • Ip
  • SDH
  • SDH-IP
  • SDH-Ip
  • SDHb
  • sdhB
  • CWS2
  • IP
  • PGL4
  • SDH1
  • SDH2
  • SDHIP
  • 0710008N11Rik
  • fj46d06
  • wu:fj46d06
Epitope
C-Term
26
16
13
12
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
91
41
27
3
3
1
1
1
1
1
1
Host
Goat
75
18
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
73
31
16
12
11
10
9
2
1
1
1
Supplier
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Immunogen Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2.
Blocking Peptide Blocking peptide for this product available: ABIN368813
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SDHB (SDHB Antibody Abstract)
Background Synonyms: SDHB, IP, SDH, SDH1, SDHIP, succinate dehydrogenase complex, subunit B, iron sulfur (Ip), OTTHUMP00000044624, iron-sulfur subunit, FLJ92337, SDH2, SDHIP
Gene ID 6390
NCBI Accession NP_002991
Research Area Metabolism
Pathways
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 28 kDa band observed in Human Liver lysates (calculated MW of 31.6 kDa according to NP_002991.1). Recommended concentration: 0.03-0.1 µg/mL. ELISA: Customer successfully used this product in quantitative ELISA on HepG2 lysates.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (C-Term) antibody (ABIN185447) ABIN185447 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). ...
Background publications Selak, Armour, MacKenzie et al.: "Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase." in: Cancer cell, Vol. 7, Issue 1, pp. 77-85, 2005 (PubMed).

Morris, Maina, Morgan et al.: "Molecular genetic analysis of FIH-1, FH, and SDHB candidate tumour suppressor genes in renal cell carcinoma." in: Journal of clinical pathology, Vol. 57, Issue 7, pp. 706-11, 2004 (PubMed).