Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (C-Term) antibody

Details for Product No. ABIN185447
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Antigen
Synonyms succinate dehydrogenase 2-3, CG3283, Dmel\\CG3283, Ip, SDH, SDH-IP, SDH-Ip, SDHb, sdhB, CWS2, IP, PGL4, SDH1, SDH2, SDHIP, 0710008N11Rik, fj46d06, wu:fj46d06
Epitope
C-Term
(11), (9), (7), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(51), (28), (19), (13), (13), (13), (1), (1), (1), (1), (1)
Host
Goat
(44), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(41), (13), (10), (10), (10), (9), (7), (5), (2), (1), (1)
Pubmed 2 references available
Quantity 100 μg
Options
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Availability Available
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Catalog No. ABIN185447
355.60 $
Plus shipping costs $45.00

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SDHB
Background Synonyms: SDHB, IP, SDH, SDH1, SDHIP, succinate dehydrogenase complex, subunit B, iron sulfur (Ip), OTTHUMP00000044624, iron-sulfur subunit, FLJ92337, SDH2, SDHIP
Gene ID 6390
NCBI Accession NP_002991
Research Area Metabolism
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 28 kDa band observed in Human Liver lysates (calculated MW of 31.6 kDa according to NP_002991.1). Recommended concentration: 0.03-0.1 µg/mL. ELISA: Customer successfully used this product in quantitative ELISA on HepG2 lysates.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (C-Term) antibody ABIN185447 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Selak, Armour, MacKenzie et al.: "Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase." in: Cancer cell, Vol. 7, Issue 1, pp. 77-85, 2005 (PubMed).

Morris, Maina, Morgan et al.: "Molecular genetic analysis of FIH-1, FH, and SDHB candidate tumour suppressor genes in renal cell carcinoma." in: Journal of clinical pathology, Vol. 57, Issue 7, pp. 706-11, 2004 (PubMed).

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