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SDHB antibody (C-Term)

Verified SDHB Reactivity: Human WB, ELISA, IHC, FACS, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN185447
  • Target See all SDHB Antibodies
    SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))
    Binding Specificity
    • 16
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 75
    • 34
    • 23
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 72
    • 4
    • 1
    Goat
    Clonality
    • 61
    • 16
    Polyclonal
    Conjugate
    • 40
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SDHB antibody is un-conjugated
    Application
    • 57
    • 23
    • 18
    • 13
    • 13
    • 11
    • 11
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Purpose
    SDHB
    Sequence
    ATYKEKKASV
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2.
    Isotype
    IgG
    Top Product
    Discover our top product SDHB Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 6-8 μg/mL.

    Western Blot: Approx 28-30 kDa band observed in lysates of cell lines HEK293, Jurkat and HeLa (calculated MW of 31.6 kDa according to NP_002991.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the plasm membrane/ cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))
    Alternative Name
    SDHB (SDHB Products)
    Background
    SDHB, IP, SDH, SDH1, SDHIP, succinate dehydrogenase complex, subunit B, iron sulfur (Ip), OTTHUMP00000044624, iron-sulfur subunit, FLJ92337, PGL4, SDH2, SDHIP
    Gene ID
    6390
    NCBI Accession
    NP_002991
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