SDHB antibody (C-Term)

Catalog No. ABIN2855873

The Rabbit Polyclonal anti-SDHB antibody is suitable to detect SDHB in samples from Human and Mouse. It has been validated for WB, IHC (p), IF and ICC.

Quick Overview for SDHB antibody (C-Term) (ABIN2855873)

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SDHB Antibody

Binding Specificity: C-Term

Cross-Reactivity: Rat (Rattus), Pig (Porcine), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rat (87 %), Pig (100 %), Rhesus Monkey (100 %), Bovine (89 %)

Characteristics: Rabbit Polyclonal antibody to SDHB (succinate dehydrogenase complex, subunit B, iron sulfur (Ip))
SDHB antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SDHB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: THP-1 , NCI-H929 , Neuro2A

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SDHB

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Alternative Name: SDHB

Background: Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.

Cellular Localization: Mitochondrion inner membrane

Molecular Weight: 32 kDa

Gene ID: 6390