Cofilin 2 (Muscle) (CFL2) antibody

Antigen: Cofilin 2 (Muscle) (CFL2)

Synonyms: NEM7, CFL2, wu:fb17d06, wu:fb18d11, wu:fd59f08, wu:fj34b08, xac2, MGC53097, MGC77288, zgc:77288, cfl2, MGC140740, cf12

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185454

Additional Information

Alternative name: Cofilin 2 (muscle)

Gene ID: 1073

Immunogen: C-DIKDRSTLGEK

Format: Affinity-purified

Description: All names and symbols: cofilin 2 (muscle) , HGNC:1875 , CFL2. Official Symbol: CFL2. RefSeq number(s): NP_068733.1, NP_619579.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579) Human, Mouse, Rat, Dog, Pig, Sheep

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 18kDa band observed in human duodenum, heart and skeletal muscle lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185454 (0.003µg/ml) staining of human muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing muscle Cofilin 2 (RC222215) and probed with ABIN185454 (mock transfection in first lane), tested by Origene.

Publications

Publications: Thirion, Stucka, Mendel et al.: "Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle." in: European journal of biochemistry / FEBS, Vol. 268, Issue 12, pp. 3473-82, 2001 (PubMed).