Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3) antibody

Antigen: Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3)

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN185456

Additional Information

Alternative name: CFC1 / Cripto-1

Gene ID: 55997

Immunogen: HFTGRYCEHDQRRSEC

Description: All names and symbols: CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family 1, cripto-1, cryptic, cryptic gene, MGC133213, CR-1. Official Symbol: CFC1. RefSeq number(s): NP_115934.1. Gene ontology terms: cellular_component unknown, determination of left/right symmetry, molecular_function unknown

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testes lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Bianco, Strizzi, Ebert et al.: "Role of human cripto-1 in tumor angiogenesis." in: Journal of the National Cancer Institute, Vol. 97, Issue 2, pp. 132-41, 2005 (PubMed).