Rab11 Family-Interacting Protein 1 (RAB11FIP1) antibody

Antigen: Rab11 Family-Interacting Protein 1 (RAB11FIP1)

Synonyms: RCP, NOEL1A, FLJ22524, FLJ22622, MGC78448, rab11-FIP1, DKFZp686E2214, RGD1562965, Rab11-FIP1, MGC122989

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185459

Additional Information

Alternative name: RAB11FIP1

Gene ID: 80223

Immunogen: C-EATKEAKESKKPE

Description: All names and symbols: RAB11FIP1, RAB11 family interacting protein 1 (class I) , HGNC:30265, FLJ22524, FLJ22622, MGC78448, NOEL1A, RCP, rab11-FIP1 , Rab coupling protein, Rab effector protein, Rab-interacting recycling protein, Rab11-family interacting protein 1D, no Rab11-binding protein 1A . Official Symbol: RAB11FIP1. RefSeq number(s): NP_079427.3, NP_001002814.1

Specificity: This antibody is expected to recognise isoform 1 (NP_079427.3) and isoform 3 (NP_001002814.1). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 90kDa band in lysates of cell line HeLa after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 71.0kDa according to NP_079427.3. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Garcia, Pole, Chin et al.: "A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes." in: Oncogene, Vol. 24, Issue 33, pp. 5235-45, 2005 (PubMed).