TGFB-Induced Factor Homeobox 2 (TGIF2) antibody

Antigen: TGFB-Induced Factor Homeobox 2 (TGIF2)

Synonyms: C80753, C81206, 4921501K24, 5730599O09Rik, xtgif2, MGC81646, TGIF2, Tgif2, MGC188777

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185466

Additional Information

Alternative name: TGIF2

Gene ID: 60436, 228839 (mouse)

Immunogen: C-EMELQKQQDP

Format: Affinity-purified

Description: All names and symbols: TGIF2, TGFB-induced factor 2 (TALE family homeobox), homeobox protein TGIF2, 5'-TG-3' interacting factor 2, TGF(beta)-induced transcription factor 2, transcription growth factor-beta-induced factor 2. Official Symbol: TGIF2. RefSeq number(s): NP_068581.1. Gene ontology terms: nucleus, regulation of transcription, DNA-dependent, transcription factor activity

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 22kDa band observed in lysates of A431, A549 and HEK293 cell lines (calculated MW of 25.9kDa according to NP_068581.1). Recommended concentration: 0.3-1.0µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185466 (0.3 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Melhuish, Gallo, Wotton: "TGIF2 interacts with histone deacetylase 1 and represses transcription." in: The Journal of biological chemistry, Vol. 276, Issue 34, pp. 32109-14, 2001 (PubMed).