Monoamine Oxidase A (MAOA) antibody

Antigen: Monoamine Oxidase A (MAOA)

Synonyms: AA407771, MGC27811, 1110061B18Rik, Mao, MAOA, MGC142852, MAOB, DKFZp469A1517, LOC100221249

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185474

Additional Information

Alternative name: Monoamine Oxidase A

Gene ID: 4128

Immunogen: C-DAPWEAQHADKWDK

Format: Affinity-purified

Description: All names and symbols: monoamine oxidase A , HGNC:6833, MAOA, RP1-201D17__B.2. Official Symbol: MAOA. RefSeq number(s): NP_000231.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in lysates of human heart and kidney fibroblast cell line HEK293 (calculated MW of 60.0kDa according to NP_000231.1). Recommended concentration: 0.1-0.3µg/ml. An additional faint band of appox 85kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology

Restrictions: For Research Use only

Images

ABIN185474 (0.3µg/ml) staining of human heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Domschke, Sheehan, Lowe et al.: "Association analysis of the monoamine oxidase A and B genes with attention deficit hyperactivity disorder (ADHD) in an Irish sample: preferential transmission of the MAO-A 941G allele to affected children." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 134B, Issue 1, pp. 110-4, 2005 (PubMed).