Adenosine A2b Receptor (ADORA2B) antibody

Antigen: Adenosine A2b Receptor (ADORA2B)

Synonyms: ADORA2B, MGC138933, si:dkey-13b16.1, LOC606745, LOC100220469, ADORA2, A2b, A2BR, A2BAR, AA2BR, AI480866, AI605384, MGC144574, MGC144575

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185477

Additional Information

Alternative name: Adenosine A2b Receptor

Gene ID: 136

Immunogen: CQADVKSGNGQ

Format: Affinity-purified

Description: All names and symbols: adenosine A2b receptor, ADORA2B, HGNC:264, ADORA2, netrin-1 receptor. Official Symbol: ADORA2B. RefSeq number(s): NP_000667.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments in Human Colon, Lung and Placenta lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules . Recommended concentration, 3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cell Cycle, DNA/RNA

Restrictions: For Research Use only

Images

ABIN185477 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Bazzichi, Trincavelli, Rossi et al.: "A2B adenosine receptor activity is reduced in neutrophils from patients with systemic sclerosis." in: Arthritis research & therapy, Vol. 7, Issue 2, pp. R189-95, 2005 (PubMed).