Oxysterol Binding Protein-Like 10 (OSBPL10) antibody

Antigen: Oxysterol Binding Protein-Like 10 (OSBPL10)

Synonyms: OPR-10, 4933433D06Rik, C820004B04Rik, ORP10, OSBP9, FLJ20363, RGD1561652

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185497

Additional Information

Alternative name: OSBPL10

Gene ID: 114884

Immunogen: C-DAATEQKRHLEEKQ

Format: Affinity-purified

Description: All names and symbols: OSBPL10, oxysterol binding protein-like 10 [Homo sapiens] , HGNC:16395, FLJ20363, ORP10, OSBP9 , OSBP-related protein 10, oxysterol-binding protein-like protein 10, oxysterol-binding protein-related protein 10. Official Symbol: OSBPL10. RefSeq number(s): NP_060254.2. Gene ontology terms: Obesity

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 90kDa band observed in human duodenum and human testes lysates (calculated MW of 84.0kDa according to NP_060254.2). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Images

ABIN185497 (1µg/ml) staining of human duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Wang, Weng, Anderson: "OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation." in: Science (New York, N.Y.), Vol. 307, Issue 5714, pp. 1472-6, 2005 (PubMed).