SH2 Domain Containing 1A (SH2D1A) antibody

Antigen: SH2 Domain Containing 1A (SH2D1A)

Synonyms: LYP, SAP, XLP, DSHP, EBVS, IMD5, XLPD, MTCP1, FLJ18687, FLJ92177

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185514

Additional Information

Alternative name: SH2D1A/SLAM associated protein

Gene ID: 4068

Immunogen: C-QYPVEKKSSARSTQ

Format: Affinity-purified

Description: All names and symbols: SH2D1A, SH2 domain protein 1A, Duncan's disease (lymphoproliferative syndrome), HGNC:10820, DSHP, EBVS, IMD5, LYP, MTCP1, SAP, XLP, XLPD , SH2 domain protein 1A, SLAM (signaling lymphocyte activation molecule) associated protein, T cell signal transduction molecule SAP. Official Symbol: SH2D1A. RefSeq number(s): NP_002342.1

Specificity: Human 128 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 16kDa band observed in human lymph node lysates (calculated MW of 14.2kDa according to NP_002342.1). Recommended concentration: 0.2-0.6µg/ml. Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185514 (0.2µg/ml) staining of human lymph node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Romero, Benitez, March et al.: "Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4)." in: Tissue antigens, Vol. 64, Issue 2, pp. 132-44, 2004 (PubMed).