Ribosomal Protein S19 (RPS19) antibody

Antigen: Ribosomal Protein S19 (RPS19)

Synonyms: DBA

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185519

Additional Information

Alternative name: RPS19

Gene ID: 6223

Immunogen: C-KMVEKDQDGGRK

Description: All names and symbols: RPS19, ribosomal protein S19, HGNC:10402, DBA , 40S ribosomal protein S19. Official Symbol: RPS19. RefSeq number(s): NP_001013.1

Specificity: Human, Mouse, Rat, Dog, Cat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 15kDa band observed in Human lysates of cell lines Daudi and K562 (calculated MW of 16.1kDa according to NP_001013.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

ABIN185519 (0.03µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Leger-Silvestre, Caffrey, Dawaliby et al.: "Specific Role for Yeast Homologs of the Diamond Blackfan Anemia-associated Rps19 Protein in Ribosome Synthesis." in: The Journal of biological chemistry, Vol. 280, Issue 46, pp. 38177-85, 2005 (PubMed).