phosphodiesterase 4D, CAMP-Specific (PDE4D) antibody

Antigen: phosphodiesterase 4D, CAMP-Specific (PDE4D)

Synonyms: DPDE3, STRK1, HSPDE4D, PDE4DN2, FLJ97311, DKFZp686M11213

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185530

Additional Information

Alternative name: PDE4D

Gene ID: 5144

Immunogen: QPEACVIDDRSPDT

Format: Affinity-purified

Description: All names and symbols: PDE4D, phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) , HGNC:8783, DPDE3, HSPDE4D, PDE4DN2, STRK1, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase PDE4D6, dunce-like phosphodiesterase E3, phosphodiesterase 4D, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3). Official Symbol: PDE4D. RefSeq number(s): NP_006194.2

Specificity: Please note this antibody is not expected to recognize isoform 4. Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Figure 1: detects transfected Human PDE4D1 and PDE4D3 in COS cell lysates as bands of approx. 68kDa and 95kDa respectively. Figure 2: Approx 70,+80+90kDa band observed in A549 lysates (calculated MW of 76.5kDa according to NP_006194.2). Recommended concentration: 0.1-0.3µg/ml.Recommended concentration: 1-3 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Enzymes, Metabolism

Restrictions: For Research Use only

Images

ABIN185530 staining (1µg/ml) of COS cell lysates (25µg protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185530 staining (0.3µg/ml) of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Houslay: "The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target." in: Molecular pharmacology, Vol. 68, Issue 3, pp. 563-7, 2005 (PubMed).

Staton, Sayer, Hankey et al.: "Association between phosphodiesterase 4D gene and ischaemic stroke." in: Journal of neurology, neurosurgery, and psychiatry, Vol. 77, Issue 9, pp. 1067-9, 2006 (PubMed).