Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (internal) antibody

Antigen: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Synonyms: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185543

Additional Information

Alternative name: GAPDH (Internal)

Gene ID: 2597

Immunogen: C-GVNHEKYDNSLK

Format: Affinity-purified

Description: All names and symbols: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685. Official Symbol: GAPDH. RefSeq number(s): NP_002037.2. Gene ontology terms: glucose metabolism, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity, oxidoreductase activity,

Specificity: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting. Human, Mouse, Rat, Dog, etc

Synonyms: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 36kDa band observed in Human Liver, Testes and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1kDa according to NP_002037.2). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185543 (0.1µg/ml) staining of Human Tonsil Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Ismail, Park: "Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase." in: Acta crystallographica. Section D, Biological crystallography, Vol. 61, Issue Pt 11, pp. 1508-13, 2005 (PubMed).

Carr, Vugler, Lawrence et al.: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." in: Molecular vision, Vol. 15, pp. 283-95, 2009 (PubMed).

Carr, Vugler, Yu et al.: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." in: Molecular vision, Vol. 17, pp. 1701-15, 2011 (PubMed).