Phosphodiesterase 4B (PDE4B) antibody

Antigen: Phosphodiesterase 4B (PDE4B)

Synonyms: DPDE4, PDE4B5, PDEIVB, MGC126529, DKFZp686F2182, Dpde4, dunce, R74983, pde4b, fj87g04, wu:fj87g04, PDE4B, MGC152127, MGC154987

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185574

Additional Information

Alternative name: Phosphodiesterase 4B

Gene ID: 5142

Immunogen: C-DIDIATEDKSPVDT

Description: All names and symbols: PDE4B, phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila), RP5-876C12.1, DKFZp686F2182, DPDE4, MGC126529, PDEIVB , OTTHUMP00000046896, cAMP-specific 3',5'-cyclic phosphodiesterase 4B, dunce-like phosphodiesterase E4, phosphodiesterase 4B, cAMP-specific, phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E4). Official Symbol: PDE4B. RefSeq number(s): NP_002591.2, NP_001032418.1, NP_001032416.1, NP_001032417.1

Specificity: This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1). Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 85kDa band observed in Mouse Brain lysates (calculated MW of 83.3kDa according to NP_002591.2). Recommended concentration: 0.5-1.0 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185574 (0.5µg/ml) staining of Mouse Brain Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Millar, Pickard, Mackie et al.: "DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling." in: Science (New York, N.Y.), Vol. 310, Issue 5751, pp. 1187-91, 2005 (PubMed).