Engrailed Homeobox 2 (EN2) antibody

Antigen: Engrailed Homeobox 2 (EN2)

Synonyms: EN2, en, en2

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185576

Additional Information

Alternative name: Engrailed-2

Gene ID: 2020

Immunogen: Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2

Format: Affinity-purified

Description: All names and symbols: Engrailed-2, engrailed homolog 2 , EN2, AUTS1. Official Symbol: EN2. RefSeq number(s): NP_001418.2.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx. 30kDa band observed in Mouse and Rat Brain lysates (calculated MW of 34.2kDa according to Human NP_001418.2 and of 33.8kDa according to Mouse NP_034264.1 and of 33.7kDa according to Rat NP_001102684.1). Recommended concentration: 0.1-0.3µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Images

EB07120 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Benayed, Gharani, Rossman et al.: "Support for the homeobox transcription factor gene ENGRAILED 2 as an autism spectrum disorder susceptibility locus." in: American journal of human genetics, Vol. 77, Issue 5, pp. 851-68, 2005 (PubMed).