DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A) antibody

Antigen: DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A)

Synonyms: SMIF, FLJ21691, SMAD4IP1, HSA275986, Nbla00360, Mitc1, AU019772, D14Ertd817e, 1110066A22Rik, 4930568L04Rik, RGD1561298, DCP1A, MGC159576

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185579

Additional Information

Alternative name: DCP1A

Gene ID: 55802, 75901 (mouse)

Immunogen: C-QVLTKNKDNHN

Description: All names and symbols: DCP1A, decapping enzyme , HSA275986, Nbla00360, SMAD4IP1, SMIF, Smad4-interacting transcriptional co-activator, decapping enzyme hDcp1a, putative protein product of Nbla00360, transcription factor SMIF. Official Symbol: DCP1A. RefSeq number(s): NP_060873.3

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 75kDa band observed in human lysates of A431 and HeLa (calculated MW of 63.3kDa according to NP_060873.3). Recommended concentration: 1-3µg/ml. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185579 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Cougot, Babajko, Séraphin: "Cytoplasmic foci are sites of mRNA decay in human cells." in: The Journal of cell biology, Vol. 165, Issue 1, pp. 31-40, 2004 (PubMed).