DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6)

Synonyms: P54, RCK, HLR2, FLJ36338, DDX6, RGD1564560, si:ch211-147p17.1, p54, rck, P54H, Xp54, hlr2, me31b, MGC145663

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185580

Additional Information

Alternative name: DEAD-box protein 6

Gene ID: 1656

Immunogen: C-AEYHSEPVEDEKP

Format: Affinity-purified

Description: All names and symbols: DEAD-box protien 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DDX6, HLR2, P54, RCK, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD),. Official Symbol: DDX6. RefSeq number(s): NP_004388.1. Gene ontology terms: RNA decay, P-bodies

Specificity: Human, Mouse, Rat, Dog 472 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 50kDa band observed in human T-cell line Jurkat lysates (calculated MW of 53.2kDa according to NP_004388.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185580 (0.3µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Cougot, Babajko, Seraphin: "Cytoplasmic foci are sites of mRNA decay in human cells." in: The Journal of cell biology, Vol. 165, Issue 1, pp. 31-40, 2004 (PubMed).